Gene description for Hspa1b
Gene name heat shock protein 1B
Gene symbol Hspa1b
Other names/aliases HSP70B1
Hsp70
Hsp70-1
Hsp70.1
hsp68
Species Mus musculus
 Experiment description of studies that identified Hspa1b in Breast cancer cells

1
Experiment ID1051
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name67NR
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
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2
Experiment ID1052
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name66cl4
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

3
Experiment ID1053
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

4
Experiment ID1054
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVEVs
Identification method Mass spectrometry
PubMed ID 29115712   
OrganismMus musculus
Experiment descriptionProteomic Profiling of Exosomes Secreted by Breast Cancer Cells with Varying Metastatic Potential.
AuthorsGangoda L, Liem M, Ang CS, Keerthikumar S, Adda CG, Parker BS, Mathivanan S.
Journal name Proteomics.
Publication year2017
SampleBreast cancer cells
Sample name4T1.2
Isolation/purification methodsDifferential centrifugation
Ultracentrifugation
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyMass spectrometry
Western blotting
EV-TRACK -

5
Experiment ID1996
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34112803   
OrganismMus musculus
Experiment descriptionTumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
AuthorsLima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A.
Journal name Nat Commun
Publication year2021
SampleBreast cancer cells
Sample nameEO771
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectometry
EV-TRACK -

6
Experiment ID1997
Identified moleculeprotein
Extracellular vesicle typeExosomes
MISEVSmall EVs
Identification method Western blotting
PubMed ID 34112803   
OrganismMus musculus
Experiment descriptionTumor microenvironmental cytokines bound to cancer exosomes determine uptake by cytokine receptor-expressing cells and biodistribution
AuthorsLima LG, Ham S, Shin H, Chai EPZ, Lek ESH, Lobb RJ, Müller AF, Mathivanan S, Yeo B, Choi Y, Parker BS, Möller A.
Journal name Nat Commun
Publication year2021
SampleBreast cancer cells
Sample namePyMT
Isolation/purification methodsDifferential centrifugation
Filtration
Ultracentrifugation
Size exclusion chromatography
Centrifugal ultrafiltration
Flotation density-
Molecules identified in the studyProtein
Methods used in the studyWestern blotting
Mass spectometry
EV-TRACK -

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